After 24 h incubation, the PCM was centrifuged at 1000 g for 15 min (37C), filtered using a 0
After 24 h incubation, the PCM was centrifuged at 1000 g for 15 min (37C), filtered using a 0.22-m sterile filtration system, kept and gathered at -80C until make use of. (SSM), SSM from TB Monocrotaline rats (SSM-TB), prostate-conditioned mass media (PCM) or PCM from TB rats (PCM-TB) for 24-96 … Continue reading After 24 h incubation, the PCM was centrifuged at 1000 g for 15 min (37C), filtered using a 0
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